Atp7b Knockout cell line (AML12)

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location： Home > Products > KO Cell Lines > Atp7b Knockout cell line (AML12)

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Atp7b Knockout cell line (AML12) Order

Atp7b Knockout cell line (AML12)
Catalog#
YKO-M214
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Atp7b Knockout cell line (AML12)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Atp7b Knockout cell line (AML12)
Morphology
Epithelial-like, adherent
Passage ratio
1：3~1：6
Species
Mouse
Gene Name
Atp7b
Gene ID
11979
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM/F-12+10%FBS+1%ITS Liquid Media Supplement+40ng/ml Dexamethasone
Target region
Knockout region: Exon 2 (based on transcript Atp7b-201)
Validation: PCR+Sanger Sequencing
Atp7b Knockout cell line (AML12) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:AML12		 STR Info (Cell bank)
Cell Line:AML12
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
4-2 238.09
【20.3】		238.08
【20.3】
5-5 335.69
【14】		339.78
【15】		335.95
【14】		339.97
【15】
6-4 290.87
【16】		290.82
【16】
6-7 334.62
【12】		334.65
【12】
9-2 221.52
【15】		221.6
【15】
12-1 238.45
【19】		238.52
【19】
15-3 197.06
【21.3】		197.23
【21.3】
18-3 172.81
【21】		172.91
【21】
X-1 404.71
【26】		405.34
【26】
D4S2408
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Atp7b gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the AML12 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Atp7b gene knockout Atp7b Knockout cell line (AML12) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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